Application of microporous filtration membrane in biological functional proteins
Issuing time:2023-11-20 15:17
Microcellulose filter membranes play an important role in the separation, purification and concentration of biologically functional proteins. These filter membranes, which are composed of countless small pores, in addition to screening, also interact with protein molecular properties, including the following aspects :
1. The filtration mechanism of the membrane. The microporous filtration membrane is composed of a network of micropores that act as a sieve, allowing smaller molecules to pass while retaining larger molecules (such as proteins). Therefore, the pore size of the membrane is important for determining protein separation. The molecular size cutoff is critical, large proteins cannot pass through the membrane and are separated, while small proteins do cross the membrane and enter aqueous solution, a process that strongly depends on the molecular weight of the protein.
2. Size exclusion. Microporous membranes mainly use size exclusion as a separation mechanism. Proteins smaller than the pore size can freely pass through the pores and be collected as permeates. However, larger proteins cannot enter the pores and remain in the membrane as retentates. Ostensibly, this molecular sieving effect separates proteins based on molecular size.
3. Protein-membrane interaction. From the beginning of membrane filtration, protein-membrane interaction plays an important role in the filtration process, because the entire process may be affected by factors such as charge, hydrophobicity, and surface chemistry of proteins and membranes. Electrostatic Molecular forces such as interactions, hydrogen bonds, and van der Waals forces come into play, which directly affect protein penetration and retention.
4. Adsorption and fouling. Adsorption refers to the adhesion of proteins to the membrane surface, which will affect the filtration efficiency. The protein may undergo reversible or irreversible adsorption, depending on the strength of the protein-membrane interaction. Irreversible adsorption can lead to fouling. Over time, proteins will accumulate on the membrane surface, reducing permeability and affecting separation performance. Therefore, the molecular mechanisms of protein adsorption and fouling must be taken into consideration at the beginning of membrane selection.
5. Selectivity and protein purification, microporous membranes provide selectivity for protein separation based on size exclusion. By controlling the pore size and membrane characteristics, specific proteins can be separated. Smaller impurities and molecules can pass through the pores, while larger features Sexual proteins are retained, and this selectivity allows the purification of biologically functional proteins from complex mixtures.
6. Membrane surface modification. Surface modification of microporous membranes can enhance protein separation. Surface modification can change the charge, hydrophilicity/hydrophobicity and chemical function of the membrane surface, thereby affecting protein-membrane interactions. Coating the membrane with polymers Strategies such as modifying the surface with specific functional groups can improve selectivity, reduce fouling, and enhance overall filtration performance.
In short, the application of microcellulose filter membrane interacts with the molecular properties of proteins, which can effectively separate and purify the required proteins, and can realize the efficient separation and purification process of biological functional proteins, thus improving the quality and purity of the final product.